elisa antibody:Anti-Cyfra21-1(CK19)(Coating) antib

SPECIFICATION SHEETAnti-Cyfra21-1(CK19)(Coating) antibodyCatalog #: L1C00701 Lot #: C080301 Description: MAb to Cyfra21-1(CK19) Specificity: Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Polypeptide reacting: Mr 40 000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQLA) of the α-helical fragment. Tumors specifically detected: All tested adenocarcinoma; cholangio ca. of liver; renal cell carcinoma; transitional cell ca. of the bladder; ovary ca.; squamous cell ca. of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast ca.; thymoma Clone: KS19.1 (1604) Host Animal: Mouse. Hybridization of P3-X63-Ag8.653 .myeloma cells with spleen cells from Balb/c mice. Isotype: IgG2a, Source: Ascites Immunogen: Keratin K19 of Mr 40 000; from cultured human MCF-7 cells Format: Purified, Liquid Purification: >90% pure (SDS-PAGE). Protein A chromatography. Concentration: 2mg/ml (0.7×OD280nm) Affinity Constant: 2x 10-10 mol/l. Buffer: 10mM Sodium Phosphate, 0.15M Sodium Chloride, pH 7.4 Preservative: none Applications: Immunohistochemistry on frozen or paraffin sections. Each laboratory should determine an optimum working titer for use in its particular application. Recommended antibody pair for immunoassay: Capture Detection L1C00701 L1C00702 Storage: Upon receipt, aliquot and store at <–40°C. Avoid multiple freeze/thaw cycles. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used.